Overexpression of B7-H3 in CD133+ colorectal cancer cells is associated with cancer progression and survival in human patients.

Abstract

Cancer stem-like cells are enriched in CD133-positive (CD133(+)) colorectal cancer (CRC) cells. To date, the biological significance of CD133 expression in cancer stem-like cells is still unknown. B7-H3, a costimulatory molecule, plays a pivotal role in tumor immune escape by inhibiting the functions of T cells. To identify a new marker to predict the tumor grade of CRC, we analyzed the expression of B7-H3 and CD133 in colorectal tumor samples, and their clinical significance was determined. By using a series of techniques including pathologic tissue microarray technology, immunohistochemistry, and immunofluorescent staining, we found B7-H3 was expressed in 56.73% of the CRC cases (59/104) sampled; CD133 was detected in 26.92% of the CRC cases (28/104) sampled. Further analysis indicated that 22 of these CD133(+) samples expressed B7-H3. We also found coexpression of CD133 and B7-H3 in tumor tissue samples (r = 0.321, P < 0.01). Moreover, in contrast to individual CD133 or B7-H3 expression, the coexpression of B7-H3 and CD133 was evidently associated with the depth of tumor invasion, lymphatic metastasis, distant metastasis, and Dukes' stage, suggesting it is a valuable biomarker for the progression of CRC. Indeed, the patients with coexpression of B7-H3 and CD133 had a poorer survival than the other patients (P < 0.05). In summary, our results reveal that B7-H3 was aberrantly expressed in CD133(+) CRC cells, and the expression level was closely associated with tumor progression.

• Authors: Zhou Bin, Zhang Guangbo, Ge Yan, Zhou Huan, Lv Desheng, Zhang Xueguang
• Journal: The Journal of surgical research (J Surg Res) Vol. 188 Issue 2 Pg. 396-403 (May 15 2014) ISSN: 1095-8673 [Electronic] United States
• PMID: 24630518 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• AC133 Antigen
• Antigens, CD
• B7 Antigens
• CD276 protein, human
• Glycoproteins
• PROM1 protein, human
• Peptides

Topics

• AC133 Antigen
• Adenocarcinoma (genetics, mortality, pathology)
• Adult
• Aged
• Aged, 80 and over
• Antigens, CD (biosynthesis, genetics)
• B7 Antigens (biosynthesis, genetics, metabolism)
• Colon (pathology)
• Colorectal Neoplasms (genetics, mortality, pathology)
• Disease Progression
• Female
• Glycoproteins (biosynthesis, genetics)
• Humans
• Lymphatic Metastasis (genetics)
• Male
• Middle Aged
• Peptides (genetics)
• Rectum (pathology)