Expression level of miR-93 in formalin-fixed paraffin-embedded tissues of breast cancer patients.

Abstract

MiR-93 is thought to be an onco-miRNA for its capabilities of enhancing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-93 expression in formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer patients. The expression of miR-93 was examined in 101 breast cancer patients and 40 controls using real-time quantitative PCR. We found that miR-93 was markedly upregulated in breast cancer patients compared with controls (p<0.01). The expression level of miR-93 was significantly correlated with miR-24/378 in breast cancer patients. MiR-93 exhibited great capability of discriminating between cancer patients and cancer-free controls by receiver-operator characteristic (ROC) curve analysis. MiR-93 showed 0.866 AUC (the area under the ROC curve) values. The MiR-93 level was found significantly correlated with breast cancer by univariable logistic regression. These results suggest that overexpression of miR-93 in FFPE tissues may serve as an indispensable source for biomarker discovery and validation in breast cancer patients.

• Authors: Zhao-qun Deng, Jun Qian, Feng-qiong Liu, Jiang Lin, Rui Shao, Jia-yu Yin, Qin Tang, Ming Zhang, Li He
• Journal: Genetic testing and molecular biomarkers (Genet Test Mol Biomarkers) Vol. 18 Issue 5 Pg. 366-70 (May 2014) ISSN: 1945-0257 [Electronic] United States
• PMID: 24606013 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• DNA Primers
• MIRN93 microRNA, human
• MicroRNAs
• Formaldehyde

Topics

• Adult
• Base Sequence
• Biomarkers, Tumor (metabolism)
• Breast Neoplasms (genetics, pathology)
• Case-Control Studies
• DNA Primers
• Female
• Formaldehyde
• Humans
• MicroRNAs (genetics)
• Middle Aged
• Paraffin Embedding
• ROC Curve
• Real-Time Polymerase Chain Reaction
• Sensitivity and Specificity
• Up-Regulation