Immunohistochemical characterization of non-epithelial cells in spiradenoma.

Abstract

Spiradenoma is unique with respect to the presence of a large number of non-epithelial cells, including S100 protein(+) cells, most of which are presumably Langerhans cells, in the parenchyma as shown in the published work. However, the characterization of these non-epithelial cells to date is insufficient. Immunohistochemistry of CD1a, CD3, CD4, CD8, CD56, CD68, intercellular adhesion molecule-1 (ICAM-1), and HLA-DR, as well as double-immunofluorescence labeling of S100 protein/CD1a and CD1a/CD3, was performed using paraffin-embedded specimens from five cases of spiradenoma retrospectively. Non-epithelial cells evenly distributed throughout the parenchyma of spiradenoma primarily consisted of CD1a(+) Langerhans cells and CD3(+) T cells. ICAM-1 was expressed by epithelial cells and non-epithelial cells in the parenchyma. HLA-DR on the epithelial cells was limited to the focal area. In double-immunofluorescence labeling, approximately one-half of Langerhans cells were spatially related to T cells in the parenchyma, suggesting their functional interaction.

• Authors: Kohji Iida, Shinsaku Iwai, Hiroomi Hosaka, Yuki Kitami, Masaki Akiyama, Takao Suzuki, Hirohiko Sueki
• Journal: The Journal of dermatology (J Dermatol) Vol. 40 Issue 11 Pg. 896-900 (Nov 2013) ISSN: 1346-8138 [Electronic] England
• PMID: 24580131 (Publication Type: Journal Article)
• Copyright: © 2013 Japanese Dermatological Association.

Chemical References

• Antigens, CD1
• CD1a antigen

Topics

• Adenoma (immunology, pathology)
• Adult
• Aged
• Antigens, CD1 (analysis)
• Female
• Humans
• Immunohistochemistry
• Langerhans Cells (chemistry, pathology)
• Male
• Middle Aged
• Retrospective Studies
• Skin (immunology, pathology)
• Skin Neoplasms (immunology, pathology)
• T-Lymphocytes (pathology)