Abstract | CONTEXT: OBJECTIVE: The objective of the study was to determine the expression profiles of six serum miRNAs critical to atherosclerosis in SCH patients and reanalyze the association between atherosclerosis and SCH from a new perspective. OUTCOMES, DESIGN, AND PARTICIPANTS: MicroRNA profiling analysis was performed by real-time PCR in normal control subjects (NC; n = 22); patients with subclinical hypothyroidism alone (SCH; n = 20); SCH patients plus atherosclerosis (SCH+ATH; n = 21); and patients with atherosclerosis but without subclinical hypothyroidism (ATH; n = 22). RESULTS: MiR-21-5p was up-regulated in SCH, SCH+ATH, and ATH groups than in the NC group. In addition, expression levels of miR-21-5p in SCH+ATH group were higher than in SCH alone and ATH alone groups, respectively. Both miR-125a-5p and miR-126-3p showed a decreased trend from NC to SCH and then to SCH+ATH or ATH subjects. MiR-221-3p and miR-222-3p were decreased in the SCH+ATH and ATH groups compared with either the NC or SCH groups. No differences were found in the levels of miR125a-5p, miR126-3p, miR221-3p, and miR222-3p between the ATH and SCH+ATH group. CONCLUSIONS: MiR-21-5p showed the most specific expression patterns in all patients with subclinical hypothyroidism (SCH and SCH+ATH groups). Down-regulation of miR-125a-5p, miR-126-3p, miR-221-3p, and miR-222-3p may be a manifestation of atherosclerosis either in SCH+ATH or in ATH-alone patients. MiR-126-3p has the most specific expression patterns in all atherosclerosis patients (SCH+ATH and ATH groups).
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Authors | Xinhuan Zhang, Shanshan Shao, Houfa Geng, Yong Yu, Chenggang Wang, Zhanfeng Liu, Chunxiao Yu, Xiuyun Jiang, Yangxin Deng, Ling Gao, Jiajun Zhao |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 99
Issue 5
Pg. E766-74
(May 2014)
ISSN: 1945-7197 [Electronic] United States |
PMID | 24517143
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Atherosclerosis
(complications, genetics, metabolism)
- Down-Regulation
- Female
- Humans
- Hypothyroidism
(complications, genetics, metabolism)
- Male
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Real-Time Polymerase Chain Reaction
- Severity of Illness Index
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