Effect of hemoperfusion using polymyxin B-immobilized fibers on acute lung injury in a rat sepsis model.

Abstract

Direct hemoperfusion using polymyxin B-immobilized column (PMX-DHP) is recognized as an effective treatment for septic shock. However, whether its efficacy is limited to cardiovascular dysfunction remains unknown. Therefore, we planned to examine the effects of PMX-DHP in an acute lung injury model. [Materials and methods] Rats were assigned to either PMX-DHP group or control group (n= 7 in each). A lung injury was created by the intratracheal instillation of LPS. In PMX-DHP group, an arteriovenous extracorporeal circuit using PMX column was applied for three hours. The same procedure using a dummy column was applied in control group. The lung microcirculation was observed, and adherent leukocytes, RBC velocity, and the arterial PaO2 were calculated. Pathological changes and the wet/dry weight ratio of the lungs were examined. [Results] Adherent leukocytes and platelets to the lung venules were recognized at 3 hours, and their numbers increased over time. Treatment with PMX-DHP significantly suppressed these events and helped maintenance of the blood flow and PaO2 levels. The lung edema and the histologic damages were also suppressed. [Conclusions] PMX-DHP improved the microcirculation by suppressing leukocyte and platelet adhesion. PMX-DHP had beneficial effects in a model for acute lung injury.

• Authors: Toshiaki Iba, Isao Nagaoka, Atsushi Yamada, Masataka Nagayama, Takahiro Miki
• Journal: International journal of medical sciences (Int J Med Sci) Vol. 11 Issue 3 Pg. 255-61 ( 2014) ISSN: 1449-1907 [Electronic] Australia
• PMID: 24516349 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Lipopolysaccharides
• Polymyxin B

Topics

• Acute Lung Injury (chemically induced, physiopathology, therapy)
• Animals
• Anti-Bacterial Agents (therapeutic use)
• Disease Models, Animal
• Erythrocytes (pathology)
• Hemoperfusion
• Humans
• Leukocytes (pathology)
• Lipopolysaccharides (toxicity)
• Microcirculation (drug effects)
• Platelet Adhesiveness (drug effects)
• Polymyxin B (chemistry, therapeutic use)
• Rats
• Sepsis (chemically induced, physiopathology, therapy)