Abstract | INTRODUCTION: METHODS: ScFv(anti-LIBS) and control antibody mut-scFv were reacted with N-succinimidyl-4-[(18)F]fluorobenzoate (S[(18)F]FB). Radiolabeled scFv was incubated with in vitro formed platelet clots and injected into mice with FeCl(3) induced thrombus in the left carotid artery. Clots were imaged in the PET scanner and amount of radioactivity measured using an ionization chamber and image analysis. Assessment of vessel injury as well as the biodistribution of the radiolabeled scFv was studied. RESULTS: After incubation with increasing concentrations of (18)F-scFv(anti-LIBS) clots had retained significantly higher amounts of radioactivity compared to clots incubated with radiolabeled (18)F-mut-scFv (13.3 ± 3.8 vs. 3.6 ± 1 KBq, p < 0.05, n = 9, decay corrected). In the in vivo experiments we found an high uptake of the tracer in the injured vessel compared with the non-injured vessel, with 12.6 ± 4.7% injected dose per gram (ID/g) uptake in the injured vessel and 3.7 ± 0.9% ID/g in the non-injured vessel 5 minutes after injection (p < 0.05, n = 6). CONCLUSIONS: Our results show that the novel antibody radiotracer (18)F-scFv(anti-LIBS) is useful for the sensitive detection of activated platelets and thrombosis. ADVANCES IN KNOWLEDGE AND IMPLICATIONS FOR PATIENT CARE: We describe the first (18)F variant of a scFv(anti-LIBS) against activated platelets. This diagnostic agent could provide a powerful tool for the assessment of acute thrombosis and inflammation in patients in the future.
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Authors | Katie Ardipradja, Shinn Dee Yeoh, Karen Alt, Graeme O'Keefe, Angela Rigopoulos, David W Howells, Andrew M Scott, Karlheinz Peter, Uwe Ackerman, Christoph E Hagemeyer |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 41
Issue 3
Pg. 229-37
(Mar 2014)
ISSN: 1872-9614 [Electronic] United States |
PMID | 24440583
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- Benzoates
- Fluorine Radioisotopes
- Single-Chain Antibodies
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Topics |
- Animals
- Benzoates
(chemistry)
- Blood Coagulation
- Carotid Artery Thrombosis
(diagnostic imaging, pathology, physiopathology)
- Disease Models, Animal
- Female
- Fluorine Radioisotopes
- Image Processing, Computer-Assisted
- Mice
- Mice, Inbred C57BL
- Platelet Activation
- Positron-Emission Tomography
- Single-Chain Antibodies
(chemistry, pharmacokinetics)
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