Abstract | BACKGROUND: OBJECTIVES: To identify the bacteria and fungi present in cerebrospinal fluid from children with cerebrospinal fluid shunt infection using a high-throughput sequencing approach, and to compare those results to those from negative controls and conventional culture. METHODS: This descriptive study included eight children ≤18 years old undergoing treatment for culture-identified cerebrospinal fluid shunt infection. After routine aerobic culture of each cerebrospinal fluid sample, deoxyribonucleic acid ( DNA) extraction was followed by amplification of the bacterial 16S rRNA gene and the fungal ITS DNA region tag-encoded FLX- Titanium amplicon pyrosequencing and microbial phylogenetic analysis. RESULTS: The microbiota analyses for the initial cerebrospinal fluid samples from all eight infections identified a variety of bacteria and fungi, many of which did not grow in conventional culture. Detection by conventional culture did not predict the relative abundance of an organism by pyrosequencing, but in all cases, at least one bacterial taxon was detected by both conventional culture and pyrosequencing. Individual bacterial species fluctuated in relative abundance but remained above the limits of detection during infection treatment. CONCLUSIONS:
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Authors | Tamara D Simon, Christopher E Pope, Samuel R Browd, Jeffrey G Ojemann, Jay Riva-Cambrin, Nicole Mayer-Hamblett, Margaret Rosenfeld, Danielle M Zerr, Lucas Hoffman |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 1
Pg. e83229
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24421877
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Bacteria
(genetics)
- Cerebrospinal Fluid Shunts
(adverse effects)
- Communicable Diseases
(diagnosis, etiology, microbiology)
- Fungi
(genetics)
- Genetic Variation
- Humans
- RNA, Ribosomal, 16S
(genetics)
- Sequence Analysis, DNA
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