Influence of HLA-G polymorphisms in human immunodeficiency virus infection and hepatitis C virus co-infection in Brazilian and Italian individuals.

Abstract

OBJECTIVE:

This study aimed to investigate the role of Human Leukocyte Antigen (HLA)-G in the susceptibility to HIV-1 infection through the analysis of the HLA-G 3' untranslated region (UTR) polymorphisms 14 bp insertion/deletion (rs66554220) and +3142C>G (rs1063320).

DESIGN:

We analyzed 582 HIV-1 infected patients and 626 uninfected individuals from Brazil and Italy in a case-control study.

METHODS:

HLA-G polymorphisms were genotyped using PCR, PCR-RFLP assays or direct sequencing. All analyses were stratified by ethnicity. Genotypic, allelic and diplotypic frequencies were compared between HIV-1 infected subjects and controls using Chi-square or Fischer exact tests. Also, haplotypic frequencies were estimated using MLocus software.

RESULTS:

African-derived HIV-infected individuals presented a higher frequency of the 14 bp insertion allele as compared to non-infected individuals (0.468 versus 0.373, respectively; p(Bonf) = 0.010). A higher frequency of the 14 bp insertion +3142G (insG) haplotype (0.456 versus 0.346, p<0.001) and the insG/insG diplotype (OR=1.88, 95%CI = 1.08-3.23, p=0.021) was observed among African-derived patients as compared to uninfected controls. Also, we observed a higher frequency of the ins/ins genotype among African-derived HIV patients co-infected with HCV (OR=2.78, 95%CI = 1.20-6.49, p = 0.008).

CONCLUSIONS:

Our data point out to an increased frequency of alleles and genotypes associated with low HLA-G expression among African-derived patients, suggesting a potential role for HLA-G in the susceptibility to HIV-1 infection and HCV co-infection in those individuals.

• Authors: G K da Silva, Priscila Vianna, Tiago Degani Veit, Sergio Crovella, Eulalia Catamo, Elvira Alicia Aparicio Cordero, Vanessa Suñé Mattevi, Rosmeri Kuhmmer Lazzaretti, Eduardo Sprinz, Regina Kuhmmer, José Artur Bogo Chies
• Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (Infect Genet Evol) Vol. 21 Pg. 418-23 (Jan 2014) ISSN: 1567-7257 [Electronic] Netherlands
• PMID: 24389119 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• 3' Untranslated Regions
• HLA-G Antigens

Topics

• 3' Untranslated Regions
• Adolescent
• Adult
• Aged
• Black People (genetics)
• Brazil (epidemiology)
• Coinfection
• Gene Frequency
• Genetic Variation
• HIV Infections (ethnology, immunology, virology)
• HLA-G Antigens (genetics)
• Haplotypes
• Hepatitis C (ethnology, immunology, virology)
• Humans
• Italy (epidemiology)
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Young Adult