Treatment with D-penicillamine or zinc sulphate affects copper metabolism and improves but not normalizes antioxidant capacity parameters in Wilson disease.

Abstract

Copper accumulation in tissues due to a biallelic pathogenic mutation of the gene: ATP7B results in a clinical phenotype known as Wilson disease (WD). Aberrations in copper homeostasis can create favourable conditions for superoxide-yielding redox cycling and oxidative tissue damage. Drugs used in WD treatment aim to remove accumulated copper and normalise the free copper concentration in the blood. In the current study the effect of decoppering treatment on copper metabolism and systemic antioxidant capacity parameters was analyzed. Treatment naïve WD patients (TNWD) (n = 33), those treated with anti-copper drugs (TWD) (n = 99), and healthy controls (n = 99) were studied. Both TNWD and TWD patients characterised with decreased copper metabolism parameters, as well as decreased total antioxidant potential (AOP), glutathione (GSH) level, activity of catalase, glutathione peroxidase (GPx), and S-transferase glutathione, compared to controls. TWD patients had significantly lower copper metabolism parameters, higher total AOP and higher levels of GSH than TWD individuals; however, no difference was observed between these two patient groups with respect to the rest of the antioxidant capacity parameters. Patients who had undergone treatment with D-penicillamine or zinc sulphate did not differ with respect to copper metabolism or antioxidant capacity parameters, with the exception of GPx that was lower in D-penicillamine treated individuals. These data suggest that anti-copper treatment affects copper metabolism as well as improves, but does not normalize, natural antioxidant capacity in patients with WD. We propose to undertake studies aimed to evaluate the usefulness of antioxidants as well as selenium as a supplemental therapy in WD.

• Authors: Grażyna Gromadzka, Gromadzka Grażyna, Agata Karpińska, Karpińska Agata, Adam Przybyłkowski, Przybyłkowski Adam, Tomasz Litwin, Litwin Tomasz, Agata Wierzchowska-Ciok, Wierzchowska-Ciok Agata, Karolina Dzieżyc, Dzieżyc Karolina, Grzegorz Chabik, Chabik Grzegorz, Anna Członkowska, Członkowska Anna
• Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (Biometals) Vol. 27 Issue 1 Pg. 207-15 (Feb 2014) ISSN: 1572-8773 [Electronic] Netherlands
• PMID: 24368744 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Zinc Sulfate
• Copper
• Penicillamine

Topics

• Adult
• Antioxidants (metabolism)
• Copper (metabolism)
• Female
• Hepatolenticular Degeneration (diagnosis, drug therapy, metabolism)
• Humans
• Male
• Penicillamine (pharmacology, therapeutic use)
• Zinc Sulfate (pharmacology, therapeutic use)