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Hydroxyindole-O-methyltransferase in Y-79 human retinoblastoma cells: effect of cell attachment.

Abstract
Hydroxyindole-O-methyltransferase (HIOMT), the enzyme in the final step of melatonin synthesis, is present in the Y-79 human retinoblastoma cell line. Using electroblot immunolabellings, a single band corresponding to HIOMT was observed. Immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantification of enzyme activity all revealed dramatic increases in HIOMT in cells attached to substrate compared to cells in suspension culture.
AuthorsA P Kyritsis, A F Wiechmann, D Bok, G J Chader
JournalJournal of neurochemistry (J Neurochem) Vol. 48 Issue 5 Pg. 1612-6 (May 1987) ISSN: 0022-3042 [Print] England
PMID2435851 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Acetylserotonin O-Methyltransferase
Topics
  • Acetylserotonin O-Methyltransferase (metabolism)
  • Animals
  • Cattle
  • Cell Line
  • Cytosol (enzymology)
  • Eye Neoplasms (enzymology, pathology)
  • Fluorescent Antibody Technique
  • Humans
  • Molecular Weight
  • Retina (enzymology, ultrastructure)
  • Retinoblastoma (enzymology, pathology)
  • Staining and Labeling

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