Abstract | BACKGROUND: METHODS: Peripheral blood mononuclear cells were isolated from whole blood of HCC patients and stimulated to transform into dendritic cells (DCs). These DCs were then transfected with RNA extracted from HepG-2 hepatoma cells to induce expression of specific antigens. RESULTS: The transfected DCs stimulated T lymphocytes to produce cytotoxic T lymphocytes, which specifically attacked HepG-2 cells. Injection of T lymphocytes from HCC patients and transfected DCs into severe combined immunodeficiency mice limited the growth of HepG-2 tumors. CONCLUSION: A specific immune response against hepatoma can be generated in vivo by administering DCs transfected with RNA from a specific tumor. This method may have therapeutic application in humans to reduce recurrence of HCC.
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Authors | B H Xie, J Y Yang, H P Li, B Zhang, W Chen, B Zhou, B G Peng, L J Liang, Q He |
Journal | Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
(Clin Transl Oncol)
Vol. 16
Issue 8
Pg. 753-60
(Aug 2014)
ISSN: 1699-3055 [Electronic] Italy |
PMID | 24338510
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- Cancer Vaccines
- RNA, Neoplasm
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Topics |
- Animals
- Antigens, Neoplasm
(immunology)
- Cancer Vaccines
(immunology)
- Carcinoma, Hepatocellular
(immunology)
- Dendritic Cells
(immunology, transplantation)
- Flow Cytometry
- Humans
- In Vitro Techniques
- Liver Neoplasms
(immunology)
- Mice
- RNA, Neoplasm
(immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Transfection
- Xenograft Model Antitumor Assays
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