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Dendritic cells transfected with hepatocellular carcinoma (HCC) total RNA induce specific immune responses against HCC in vitro and in vivo.

AbstractBACKGROUND:
Immunotherapy is an effective method for preventing metastasis and recurrence of carcinoma. Hepatocellular carcinoma (HCC) is a common malignancy with a high rate of recurrence, and has not successfully been introduced to immunotherapy.
METHODS:
Peripheral blood mononuclear cells were isolated from whole blood of HCC patients and stimulated to transform into dendritic cells (DCs). These DCs were then transfected with RNA extracted from HepG-2 hepatoma cells to induce expression of specific antigens.
RESULTS:
The transfected DCs stimulated T lymphocytes to produce cytotoxic T lymphocytes, which specifically attacked HepG-2 cells. Injection of T lymphocytes from HCC patients and transfected DCs into severe combined immunodeficiency mice limited the growth of HepG-2 tumors.
CONCLUSION:
A specific immune response against hepatoma can be generated in vivo by administering DCs transfected with RNA from a specific tumor. This method may have therapeutic application in humans to reduce recurrence of HCC.
AuthorsB H Xie, J Y Yang, H P Li, B Zhang, W Chen, B Zhou, B G Peng, L J Liang, Q He
JournalClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico (Clin Transl Oncol) Vol. 16 Issue 8 Pg. 753-60 (Aug 2014) ISSN: 1699-3055 [Electronic] Italy
PMID24338510 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Neoplasm
  • Cancer Vaccines
  • RNA, Neoplasm
Topics
  • Animals
  • Antigens, Neoplasm (immunology)
  • Cancer Vaccines (immunology)
  • Carcinoma, Hepatocellular (immunology)
  • Dendritic Cells (immunology, transplantation)
  • Flow Cytometry
  • Humans
  • In Vitro Techniques
  • Liver Neoplasms (immunology)
  • Mice
  • RNA, Neoplasm (immunology)
  • T-Lymphocytes, Cytotoxic (immunology)
  • Transfection
  • Xenograft Model Antitumor Assays

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