Pyrimethamine-loaded lipid-core nanocapsules to improve drug efficacy for the treatment of toxoplasmosis.

Abstract

We propose an innovative product based on the nanoencapsulation of pyrimethamine (PYR), aiming an improvement of drug efficacy for the treatment of toxoplasmosis. The in vitro cytotoxicity effect of encapsulated PYR and PYR-colloidal suspension was concomitantly evaluated against LLC-MK2 lineage and mouse peritoneal macrophage showing that the cells had similar tolerance for both PYR encapsulated or in the aqueous suspension. CF1 mice acutely infected with tachyzoites of Toxoplasma gondii RH strain treated with different doses (5.0-10 mg/kg/day) of PYR-nanocapsules had survival rate higher than the animals treated with the same doses of non-encapsulated PYR. Thus, encapsulation of PYR improved the efficacy of this drug against an acute model of toxoplasmosis in mice and can be considered an alternative for reducing the dose of PYR, which, in turn, could also reduce the side effects associated to the treatment.

• Authors: Kenia Pissinate, Érica dos Santos Martins-Duarte, Scheila Rezende Schaffazick, Catiúscia Padilha de Oliveira, Rossiane Cláudia Vommaro, Sílvia Stanisçuaski Guterres, Adriana Raffin Pohlmann, Wanderley de Souza
• Journal: Parasitology research (Parasitol Res) Vol. 113 Issue 2 Pg. 555-64 (Feb 2014) ISSN: 1432-1955 [Electronic] Germany
• PMID: 24292545 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Coccidiostats
• Lipids
• Nanocapsules
• Pyrimethamine

Topics

• Animals
• Cell Line
• Cell Survival (drug effects)
• Coccidiostats (administration & dosage, therapeutic use, toxicity)
• Female
• Lipids
• Macrophages, Peritoneal (drug effects, physiology)
• Mice
• Nanocapsules
• Pyrimethamine (administration & dosage, therapeutic use, toxicity)
• Toxoplasma (drug effects)
• Toxoplasmosis, Animal (drug therapy, parasitology)