Demonstration of Epstein-Barr virus in malignant non-Hodgkin's lymphomas.

Abstract

Lymph nodes or tumor biopsies of 60 persons suspected of having a malignant lymphoma were examined for the presence of Epstein-Barr virus nuclear antigen (EBNA) by anticomplement immunofluorescence. In 8 cases the tissue specimens were also assayed for Epstein-Barr virus (EBV) DNA by nucleic acid hybridization. Serum samples of patients and controls were tested for EBV-related antibodies. The histological tests in 37 cases showed a malignant non-Hodgkin lymphoma, and in 23 cases a reactive lymphadenopathy. A Burkitt lymphoma of a European boy and a polymorphic centroblastoma contained EBNA and approximately 27 or 30 genome equivalents EBV DNA per cell, respectively. EBNA was also demonstrated in about 20% of the cells of a lymph node from a patient with recurrent reactive lymphadenopathy.

• Authors: P Wutzler, I Färber, A Sauerbrei, B Helbig, K Wutke, K D Rüdiger, K Scheibner, B Brichàcek, V Vonka
• Journal: Oncology (Oncology) Vol. 43 Issue 4 Pg. 224-9 ( 1986) ISSN: 0030-2414 [Print] Switzerland
• PMID: 2425318 (Publication Type: Case Reports, Journal Article)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• DNA, Viral

Topics

• Adolescent
• Adult
• Antibodies, Viral (analysis)
• Antigens, Viral (analysis)
• Burkitt Lymphoma (microbiology)
• Child
• DNA, Viral (analysis)
• Female
• Fluorescent Antibody Technique
• Head and Neck Neoplasms (microbiology)
• Herpesvirus 4, Human (genetics, immunology)
• Histocytochemistry
• Humans
• Lymph Nodes (metabolism)
• Lymphoma (microbiology, mortality)
• Male
• Middle Aged
• Staining and Labeling
• Tumor Virus Infections