Abstract |
Human adenoviruses possess oncogenic capacity which is well documented in mammalian animal models, but their possible implication in human malignancy has remained enigmatic. Following primary infection, adenoviruses can persist in a latent state in lymphocytes where the virus is apparently able to evade immune surveillance. In the present study, we have employed a broad-spectrum adenovirus polymerase chain reaction (PCR) assay to systematically screen more than 200 diagnostic specimens of different lymphoid malignancies including acute lymphocytic leukaemia (n=50), chronic lymphocytic leukaemia (n=50), various types of malignant lymphoma (n=100) and multiple myeloma (n=11) for the presence of adenoviral sequences. While most entities analysed revealed negative findings in virtually all specimens tested, adenoviral DNA was detected in 15/36 (42%) mantle cell lymphomas investigated. The most prevalent adenoviral species detected was C, and less commonly B. Adenovirus-positive findings in patients with mantle cell lymphoma were made at different sites including bone marrow (n=7), intestine (n=5), lymph nodes (n=2) and tonsillar tissue (n=1). The presence of adenoviral sequences identified by PCR was confirmed in individual cells by fluorescence in-situ hybridisation (FISH). The frequent observation of adenoviruses in mantle cell lymphoma is intriguings, and raises questions about their possible involvement in the pathogenesis of this lymphoid malignancy.
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Authors | Karin Kosulin, Margit Rauch, Peter F Ambros, Ulrike Pötschger, Andreas Chott, Ulrich Jäger, Johannes Drach, Alexander Nader, Thomas Lion |
Journal | European journal of cancer (Oxford, England : 1990)
(Eur J Cancer)
Vol. 50
Issue 3
Pg. 622-7
(Feb 2014)
ISSN: 1879-0852 [Electronic] England |
PMID | 24246703
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Topics |
- Adenoviridae
(genetics, isolation & purification)
- Early Detection of Cancer
- HEK293 Cells
- Hematologic Neoplasms
(metabolism, pathology, virology)
- Humans
- In Situ Hybridization, Fluorescence
(methods)
- Lymphoma, Mantle-Cell
(metabolism, pathology, virology)
- Oncogenic Viruses
(genetics, isolation & purification)
- Polymerase Chain Reaction
(methods)
- Prevalence
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