Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy.

Abstract

The physical interaction between plasma-membrane lipids and the epidermal growth factor (EGF)-receptor was investigated on single A431 human epidermoid carcinoma cells by monitoring fluorescence resonance energy transfer (FRET) between exogeneously added fluorescein-EGF (donor) and 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine (Bodipy-PC, acceptor) using donor-photobleaching FRET-microscopy. The measured mean FRET-efficiency of 13% is indicative of such a physical interaction and exemplifies the great potential and sensitivity of time-resolved imaging fluorescence microscopy techniques for the study of lipid-receptor interactions on single cells.

• Authors: T W Gadella Jr, D J Arndt-Jovin, T M Jovin
• Journal: Journal of fluorescence (J Fluoresc) Vol. 4 Issue 4 Pg. 295-8 (Dec 1994) ISSN: 1053-0509 [Print] Netherlands
• PMID: 24233602 (Publication Type: Journal Article)