Inhibition by sakyomicin A of avian myeloblastosis virus reverse transcriptase and proliferation of AIDS-associated virus (HTLV-III/LAV).

Abstract

In the course of screening for inhibitors of reverse transcriptase, we have isolated an inhibitor from a strain of Nocardia and identified it as sakyomicin A. The antibiotic blocks avian myeloblastosis virus reverse transcriptase reaction: IC50 was ca. 30 micrograms/ml by the method employed. The drug affects proliferation of HTLV-III/LAV in HTLV-I-carrying MT-4 cells: ca. 60% inhibition was observed at an antibiotic concentration of 1.0 microgram/ml and ca. 20% inhibition at 0.1 microgram/ml, and there was no significant cytotoxicity.

• Authors: N Tanaka, T Okabe, N Tanaka, Y Take, Y Inouye, S Nakamura, H Nakashima, N Yamamoto
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 77 Issue 4 Pg. 324-6 (Apr 1986) ISSN: 0910-5050 [Print] Japan
• PMID: 2422146 (Publication Type: Journal Article)

Chemical References

• Benzoquinones
• Quinones
• Reverse Transcriptase Inhibitors
• sakyomicin A

Topics

• Avian Leukosis Virus (enzymology)
• Avian Myeloblastosis Virus (enzymology)
• Benzoquinones
• Cell Line
• Deltaretrovirus (growth & development)
• Drug Evaluation, Preclinical
• Humans
• Quinones (pharmacology)
• Reverse Transcriptase Inhibitors
• Virus Replication (drug effects)