Abstract |
To study the production of anti- platelet factor 4 (anti-PF4)/ heparin complex antibodies of Ig ( immunoglobulin) G/ IgA/ IgM using enzyme-linked immunosorbent assay (ELISA; heparin-induced thrombocytopenia [HIT] antibodies) in 79 patients undergoing cardiovascular surgery, we employed Δoptical density (OD) as a marker of HIT-antibody production. The ΔODs were calculated from the differences in the ODs using ELISA. Patient were classified into 3 ΔOD ranges: ΔOD ≥ 1.0, ΔOD ≥ 0.4 to <1.0, and ΔOD < 0.4. The underlying disease, time course of the postoperative platelet count, D-dimer level, postoperative brain magnetic resonance imaging (MRI), use of cardiopulmonary bypass and postoperative thrombocytosis were not considered for the 3 ΔOD classifications. None of the 6 patients with ΔOD ≥ 1 .0 and a positive functional assay was diagnosed with HIT due to the absence of HIT-derived thrombocytopenia. In conclusion, HIT-antibody production increased until day 7 after heparin cessation and reached a trace level on day 14. It was demonstrated that HIT-antibody production is in remission unless there is any evidence of a further increase during the second week postsurgery.
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Authors | Takefumi Matsuo, Shinya Motohashi, Keiko Wanaka, Jeanine M Walenga |
Journal | Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
(Clin Appl Thromb Hemost)
Vol. 21
Issue 2
Pg. 177-80
(Mar 2015)
ISSN: 1938-2723 [Electronic] United States |
PMID | 24203352
(Publication Type: Clinical Trial, Journal Article)
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Copyright | © The Author(s) 2013. |
Chemical References |
- Autoantibodies
- Platelet Factor 4
- Heparin
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Topics |
- Autoantibodies
(blood)
- Cardiopulmonary Bypass
(adverse effects)
- Female
- Heparin
(administration & dosage, adverse effects, pharmacokinetics)
- Humans
- Magnetic Resonance Imaging
- Male
- Platelet Factor 4
- Postoperative Complications
(blood, diagnostic imaging)
- Radiography
- Thrombocytopenia
(blood, chemically induced, diagnostic imaging)
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