Immunohistochemical recognition of human nerve sheath tumors by anti-Leu 7 (HNK-1) monoclonal antibody.

Abstract

Using relatively high dilutions of anti-Leu 7 monoclonal antibody and a four-step peroxidase-antiperoxidase (PAP) reaction in paraffin-embedded tissues, we tested the affinity of this antibody to the cells of 47 human nerve sheath tumors and 22 other tumors in which the differential diagnosis with nerve sheath neoplasms is known to arise. Of all the nerve sheath tumors studied 68%, including 80% of the schwannomas, contained anti-Leu 7-positive cells. All 22 non-schwannian neoplasms were entirely negative. Specimens of eight experimental malignant rat schwannomas were also negative for anti-Leu 7 antibody. Our findings suggest that anti-Leu 7 monoclonal antibody is a promising marker that may facilitate the differential diagnosis between human Schwann cell and non-Schwann cell neoplasms.

• Authors: E Perentes, L J Rubinstein
• Journal: Acta neuropathologica (Acta Neuropathol) Vol. 68 Issue 4 Pg. 319-24 ( 1985) ISSN: 0001-6322 [Print] Germany
• PMID: 2418621 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Myelin Proteins
• Myelin-Associated Glycoprotein

Topics

• Amputation Stumps
• Animals
• Antibodies, Monoclonal
• Antigens, Neoplasm (analysis)
• Humans
• Immunoenzyme Techniques
• Myelin Proteins (analysis)
• Myelin-Associated Glycoprotein
• Neurilemmoma (immunology)
• Neurofibroma (immunology)
• Neuroma (immunology)
• Peripheral Nervous System Neoplasms (immunology)
• Rats