Abstract | BACKGROUND: OBJECTIVE: Understanding of genetic determinants of allergic disease may lead to novel therapies in controlling allergic disease. METHODS: We conducted clinical evaluation of the rates of food allergies and anaphylaxis in patients with AD-HIES, a cohort of patients with no STAT3 mutation but with similar histories of elevated IgE and atopic dermatitis, and healthy volunteers with no history of atopy. Morphine skin prick testing, ImmunoCAP assays for allergen-specific IgE, and basophil activation were measured. A model of systemic anaphylaxis was studied in transgenic mice carrying an AD-HIES mutation. STAT3 was silenced in LAD2 and primary human mast cells to study the role of STAT3 in signaling and degranulation after IgE cross-linking. RESULTS:
Food allergies and anaphylaxis were markedly diminished in patients with AD-HIES compared with a cohort of patients with no STAT3 mutation but with similar histories of elevated IgE and atopic dermatitis. Morphine skin prick testing and basophil activation were diminished in patients with AD-HIES, whereas mice carrying an AD-HIES mutation were hyporesponsive to systemic anaphylaxis models. Rapid mast cell STAT3 serine727 phosphorylation was noted after IgE cross-linking, and inhibition of STAT3 signaling in mast cells lead to impaired FcεRI-mediated proximal and distal signaling, as well as reduced degranulation. CONCLUSION: This study serves as an example for how mutations in specific atopic pathways can lead to discrete allergic phenotypes, encompassing increased risk of some phenotypes but a relative protection from others.
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Authors | Andrea M Siegel, Kelly D Stone, Glenn Cruse, Monica G Lawrence, Ana Olivera, Mi-yeon Jung, John S Barber, Alexandra F Freeman, Steven M Holland, Michelle O'Brien, Nina Jones, Celeste G Nelson, Laura B Wisch, Heidi H Kong, Avanti Desai, Orly Farber, Alasdair M Gilfillan, Juan Rivera, Joshua D Milner |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 132
Issue 6
Pg. 1388-96
(Dec 2013)
ISSN: 1097-6825 [Electronic] United States |
PMID | 24184145
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
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Copyright | Published by Mosby, Inc. |
Chemical References |
- STAT3 Transcription Factor
- Immunoglobulin E
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Topics |
- Adolescent
- Adult
- Aged
- Animals
- Cell Degranulation
(genetics)
- Cells, Cultured
- Child
- Child, Preschool
- Dermatitis, Atopic
(genetics, immunology)
- Female
- Food Hypersensitivity
(epidemiology, genetics, immunology)
- Humans
- Immunoglobulin E
(metabolism)
- Incidence
- Infant
- Job Syndrome
(epidemiology, genetics, immunology)
- Male
- Mast Cells
(immunology)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Middle Aged
- Mutation
(genetics)
- STAT3 Transcription Factor
(genetics, physiology)
- Signal Transduction
(genetics)
- Transgenes
(genetics)
- Young Adult
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