Large scale screening of human sera for HCV RNA and GBV-C RNA.

Abstract

North Carolina locates acute HIV cases by pooled nucleic acid testing of HIV-antibody negative serum samples. Here, 224 pools of 80 HIV-negative samples (N = 17,920) were screened for viral RNA from HCV, GBV-C, and influenza A. No evidence of influenza A was found, but HCV and GBV-C were common (1.2% and 1.7% prevalence, respectively), demonstrating the utility of pooled testing in locating individuals that may remain undiagnosed otherwise. By sequencing positive pools, potential transmission clusters may be located as well.

• Authors: Jessica R Keys, Peter A Leone, Joseph J Eron, Kelcie Alexander, Myra Brinson, Ronald Swanstrom
• Journal: Journal of medical virology (J Med Virol) Vol. 86 Issue 3 Pg. 473-7 (Mar 2014) ISSN: 1096-9071 [Electronic] United States
• PMID: 24178362 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Wiley Periodicals, Inc.

Chemical References

• RNA, Viral

Topics

• Adult
• Cluster Analysis
• Flaviviridae Infections (diagnosis, epidemiology, transmission, virology)
• GB virus C (isolation & purification)
• Hepacivirus (isolation & purification)
• Hepatitis C (diagnosis, epidemiology, transmission, virology)
• Hepatitis, Viral, Human (diagnosis, epidemiology, transmission, virology)
• Humans
• Molecular Epidemiology (methods)
• North Carolina (epidemiology)
• Prevalence
• RNA, Viral (blood, genetics, isolation & purification)
• Sequence Analysis, DNA