Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG).

Abstract

The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to clinical research. We show here that metabolic labelling of patient cells with alkyne-tagged sialic-acid (SiaNAl) enables an easy and reliable readout assay for the detection of CDG occurrence. It also provides valuable clues regarding the pathological processes by assessing the distribution of sialic acid analogues within the cells.

• Authors: Jorick Vanbeselaere, Dorothée Vicogne, Gert Matthijs, Christophe Biot, François Foulquier, Yann Guerardel
• Journal: Chemical communications (Cambridge, England) (Chem Commun (Camb)) Vol. 49 Issue 96 Pg. 11293-5 (Dec 14 2013) ISSN: 1364-548X [Electronic] England
• PMID: 24153356 (Publication Type: Journal Article)

Chemical References

• Alkynes
• Fluorescent Dyes
• Monosaccharides
• Sialic Acids

Topics

• Alkynes (chemistry)
• Congenital Disorders of Glycosylation (diagnosis, metabolism, pathology)
• Fibroblasts (metabolism, pathology)
• Fluorescent Dyes (chemistry)
• Glycosylation
• Golgi Apparatus (metabolism, pathology)
• Humans
• Monosaccharides (chemistry)
• Sialic Acids (chemistry)