Sarijang is a
bamboo salt soy sauce, containing extracts of Rhynchosia nulubilis,
sulfur-fed duck, dried bark of Ulmus davidiana and Allium sativum, which has been demonstrated to exert anti-inflammatory and antitumor activity. However, the cellular and molecular mechanisms of action of sarijang have not yet been elucidated. In the present study, we investigated the pro-apoptotic effects of sarijang in an in vitro U937 human
leukemia cell model. Treatment with sarijang resulted in a concentration-dependent growth inhibition of the cells, coupled with the characteristic morphological features of apoptosis. The induction of the apoptotic cell death of the U937 cells by sarijang exhibited a correlation with the upregulation of
death receptor 4 (DR4), the downregulation of members of the
inhibitor of apoptosis protein (IAP) family, including
survivin and cellular IAP (cIAP)-1, and the cleavage of Bid. Apoptosis-inducing concentrations of sarijang also induced the activation of
caspases (caspase-3, -8 and -9), accompanied by proteolytic degradation of
poly(ADP-ribose)-polymerase, β-
catenin and
phospholipase C-γ1. However, the apoptosis induced by sarijang was significantly inhibited by z-VED-fmk, a pan-
caspase inhibitor, which demonstrated the importance of
caspases in the process. These results suggested that sarijang may be a potential chemotherapeutic agent for use in the control of U937 human
leukemia cells. Further studies are required to identify the active compounds in sarijang.