Abstract |
Biliary tract cancer (BTC) has a generally poor prognosis. Furthermore, it is difficult to distinguish BTC from benign biliary disease (BBD) with commonly used modalities. Therefore, a novel biomarker to facilitate cancer detection is highly desirable. Recent studies have reported the use of circulating microRNAs ( miRNAs) as biomarkers for cancers. The purpose of this study was to evaluate whether circulating miRNA-21 (miR-21) could be used as a biomarker for BTC. Plasma samples were obtained from 94 BTC patients, 50 healthy volunteers (HVs), and 23 BBD patients. miR-21 levels in the samples were measured by qRT-PCR. Plasma miR-21 levels in patients with BTC were significantly higher than in HVs or in patients with BBD (P < 0.0001 for both). Receiver-operator curve (ROC) curve analysis in differentiating BTC patients from HVs indicated that area under the curve (AUC), optimal sensitivity and specificity was 0.93, 85.1% and 100%, respectively, and those in differentiating BTC patients from BBD patients was 0.83, 72.3%, 91.3%, respectively. Validation of these results indicated that the negative predictive value, positive predictive value, sensitivity, specificity, and accuracy in differentiating BTC patients from HVs was 76.6%, 98.6%, 84.0%, 98.0%, and 88.9%, respectively, and those in differentiating BTC patients from BBD patients was 42.2%, 93.0%, 71.2%, 82.6%, and 72.6%, respectively. These sets of values were improved by combining miR-21 and CA19-9 measurements. Plasma miR-21 is a novel diagnostic biomarker for BTC, and may be useful in distinguishing between BTC and BBD patients.
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Authors | Tomoya Kishimoto, Hidetoshi Eguchi, Hiroaki Nagano, Shogo Kobayashi, Hirofumi Akita, Naoki Hama, Hiroshi Wada, Koichi Kawamoto, Akira Tomokuni, Yoshito Tomimaru, Koji Umeshita, Yuichiro Doki, Masaki Mori |
Journal | Cancer science
(Cancer Sci)
Vol. 104
Issue 12
Pg. 1626-31
(Dec 2013)
ISSN: 1349-7006 [Electronic] England |
PMID | 24118467
(Publication Type: Journal Article)
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Copyright | © 2013 Japanese Cancer Association. |
Chemical References |
- Biomarkers, Tumor
- CA-19-9 Antigen
- MIRN21 microRNA, human
- MicroRNAs
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Topics |
- Aged
- Biliary Tract Neoplasms
(diagnosis, genetics)
- Biomarkers, Tumor
(blood)
- CA-19-9 Antigen
(blood)
- Diagnosis, Differential
- Female
- Humans
- Male
- MicroRNAs
(blood)
- Middle Aged
- ROC Curve
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