Abstract | UNLABELLED: OBJECTIVES: To establish primary CEOT epithelial-derived cell populations, investigate the expression of enamel matrix proteins (EMPs), and identify potential ameloblastin (AMBN) and patched 1 (PTCH1) gene alterations. MATERIALS AND METHODS: A 28-year-old patient with a lesion of the posterior maxilla, radiographically characterized by a radiolucency with well-defined borders containing mixed radiopacities, agreed to participate with informed consent. The patient's biopsy confirmed the diagnosis of CEOT, and a small representative tumor fragment was ascertained for cell culture. Explant cultures were established and used to establish primary cell populations. These were analyzed for morphology, cell proliferation, mineralization activity, expression of epithelial-associated markers (qRT-PCR and immunocytochemistry), and gene mutations of AMBN or PTCH1. DNA was extracted from tumor cells and gene coding and exon-intron boundaries overlapping fragments amplified. PCR products were bidirectional DNA sequenced and compared against reference sequence. RESULTS: A CEOT cell population was established and proliferated in culture and could be maintained for several passages. Expression of EMPs, cytokeratin 14 and 17, and patched (PTCH1), as well as ALP activity, was detected. These cells also had the ability to mineralize, similar to the primary tumor. Two AMBN alterations were identified in the sample: c.1323G>A/A441A (rs7680880) and c.1344*+111delA. Two single-nucleotide polymorphisms were identified in the PTCH1 gene. CONCLUSIONS: Our data support the establishment of a CEOT-derived cell population, which expresses known epithelial-associated proteins.
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Authors | Hope M Amm, Douglas L Rollins, Changchun Ren, Juan Dong, Patricia DeVilliers, Helen Rivera, Mary MacDougall |
Journal | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
(J Oral Pathol Med)
Vol. 43
Issue 3
Pg. 183-90
(Mar 2014)
ISSN: 1600-0714 [Electronic] Denmark |
PMID | 24118390
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- AMBN protein, human
- DNA, Neoplasm
- Dental Enamel Proteins
- Keratin-14
- Keratin-17
- PTCH1 protein, human
- Patched Receptors
- Patched-1 Receptor
- Receptors, Cell Surface
- enamel matrix proteins
- Alkaline Phosphatase
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Topics |
- Adult
- Alkaline Phosphatase
(analysis)
- Calcinosis
(pathology)
- Cell Culture Techniques
- Cell Proliferation
- Cell Shape
- Cells, Cultured
- DNA, Neoplasm
(genetics)
- Dental Enamel Proteins
(analysis, genetics)
- Epithelial Cells
(pathology)
- Exons
(genetics)
- Humans
- Introns
(genetics)
- Keratin-14
(analysis)
- Keratin-17
(analysis)
- Mutation
(genetics)
- Odontogenic Tumors
(chemistry, genetics, pathology)
- Patched Receptors
- Patched-1 Receptor
- Receptors, Cell Surface
(analysis, genetics)
- Sequence Analysis, DNA
- Skin Neoplasms
(chemistry, genetics, pathology)
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