Kruppel-like factor 2 regulates dendritic cell activation in patients with acute coronary syndrome.

Abstract

OBJECTIVE:

Dendritic cells (DCs) activation is important in atherosclerosis and coronary heart disease, but the mechanisms regulating activation of dendritic cells remain largely unclear. The aim of this study was to evaluate the effect of transcription factor Kruppel-like factor 2 (KLF2) in the proinflammatory activation of DCs in acute coronary syndrome.

METHODS AND RESULTS:

In this study, the expression of CD80 and KLF2 was detected in DCs in normal health controls, patients with stable angina (SA), and acute coronary syndrome (ACS). Our study found that compared with normal control and SA, KLF2 expression in DCs is reduced in patients with ACS. Moreover, the surface expression of CD80 was increased in ACS. In vitro experiment, we found that ox-LDL could increase CD80 expression and decrease KLF2 expression. Furthermore, down-regulated KLF2 could in turn increase CD80 expression via NF-κB pathway.

CONCLUSIONS:

These observations identify KLF2 as a novel negative regulator of DC function and it may play an essential role in DC activation in ACS.

• Authors: Hongcheng Fang, Jing Lin, Lei Wang, Peiyi Xie, Xiaoyan Wang, Jianyun Fu, Wen Ai, Shaoyuan Chen, Fei Chen, Fengxiao Zhang, Yousu Su, Dazhu Li
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 32 Issue 4 Pg. 931-41 ( 2013) ISSN: 1421-9778 [Electronic] Germany
• PMID: 24107715 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 S. Karger AG, Basel

Chemical References

• B7-1 Antigen
• KLF2 protein, human
• Kruppel-Like Transcription Factors
• NF-kappa B

Topics

• Acute Coronary Syndrome (genetics, metabolism)
• Adult
• Aged
• B7-1 Antigen (metabolism)
• Blotting, Western
• Cells, Cultured
• Dendritic Cells (metabolism)
• Electrophoretic Mobility Shift Assay
• Female
• Humans
• Kruppel-Like Transcription Factors (genetics, metabolism)
• Male
• Middle Aged
• NF-kappa B (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction