[A novel and facile microchip based on nitrocellulose membrane toward efficient capture of circulating tumor cells].

Abstract

A novel and facile circulating tumor cell (CTC) microchip has been developed for the isolation and detection of cancer cells. The CTC microchip was prepared based on the nitrocellulose membrane substrate, which shows high affinity to proteins and hence can adsorb antibodies naturally. We employed non-small-cells of lung cancer NCI-H1650 as target cells and testified the high capture efficacy of the CTC microchip. Furthermore, we spiked 500 cancer cells to 1 mL healthy donor's whole blood in order to simulate the detection of CTC in patient and detected 182 cancer cells ultimately, indicating the huge application potential in the future.

• Authors: Peng Zhang, Changlong Sun, Ren Zhang, Mingxia Gao, Xiangmin Zhang
• Journal: Se pu = Chinese journal of chromatography (Se Pu) Vol. 31 Issue 6 Pg. 518-21 (Jun 2013) ISSN: 1000-8713 [Print] China
• PMID: 24063189 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Membranes, Artificial
• Collodion

Topics

• Antibodies
• Cell Line, Tumor
• Cell Separation
• Collodion
• Humans
• Membranes, Artificial
• Microchip Analytical Procedures
• Neoplastic Cells, Circulating