Abstract | BACKGROUND: METHODS AND RESULTS: SF-PreCon (consisting of 3 cycles of 15-minute exposure to 2% sevoflurane before 30 minutes of MI) decreased MI/R injury in wild-type mice ( caspase-3 activity, -29.1%; infarct size, -20.2%; and left ventricular end diastolic pressure, -33.8%). In cardiac-specific AMPKα2 dominant-negative overexpressing mice, the cardioprotective effect of SF-PreCon was largely retained ( caspase-3 activity, -26.7%; infarct size, -16.7%; and left ventricular end-diastolic pressure, -25.9%; P<0.01). In contrast, SF-PreCon failed to significantly protect Cav-3 knockout mice against MI/R injury (P>0.05). SF-PreCon significantly decreased MI/R-induced superoxide generation in wild-type (-43.6%) and AMPK dominant-negative overexpressing mice (-35.5%; P<0.01) but not in Cav-3 knockout mice. SF-PreCon did not affect nicotinamide adenine dinucleotide phosphate oxidase expression but significantly inhibited cyclooxygenase-2 expression in wild-type (-38.7%) and AMPK dominant-negative overexpressing mice (-35.8%) but not in Cav-3 knockout mice. CONCLUSIONS: We demonstrate for the first time SF-PreCon mediates cardioprotection against MI/R injury via caveolin-3-dependent cyclooxygenase-2 inhibition and antioxidative effects.
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Authors | Jianli Zhao, Feng Wang, Yanqing Zhang, Liyuan Jiao, Wayne Bond Lau, Lili Wang, Baojiang Liu, Erhe Gao, Walter J Koch, Xin-Liang Ma, Yajing Wang |
Journal | Circulation
(Circulation)
Vol. 128
Issue 11 Suppl 1
Pg. S121-9
(Sep 10 2013)
ISSN: 1524-4539 [Electronic] United States |
PMID | 24030395
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anesthetics, Inhalation
- Caveolin 3
- Cyclooxygenase 2 Inhibitors
- Methyl Ethers
- Sevoflurane
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Topics |
- Anesthetics, Inhalation
(therapeutic use)
- Animals
- Caveolin 3
(deficiency, genetics, physiology)
- Cells, Cultured
- Cyclooxygenase 2 Inhibitors
(therapeutic use)
- Ischemic Preconditioning, Myocardial
(methods)
- Male
- Methyl Ethers
(therapeutic use)
- Mice
- Mice, Knockout
- Myocardial Reperfusion Injury
(drug therapy, genetics, metabolism)
- Sevoflurane
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