Abstract | BACKGROUND: METHODS: Immunohistochemical staining and Western blot were used to examine the expressions of DLC1 and PAI-1 protein in 25 specimens normal ovarian tissues, 52 specimens of serous cystadenocarcinoma tissues and 23 specimens of mucinous cystadenocarcinoma tissues. Chi-square test, Logistic regression and Partial Correlate analysis were performed to evaluate the association between DLC1 and PAI-1 with clinicopathological characteristics. Overall survival was estimated by Kaplan-Meier curves and multivariate Cox analysis. The relationships between DLC1 and PAI-1 protein expression were analyzed by Pearson's correlation coefficient. RESULTS: The expression of DLC1 protein in ovarian carcinoma tissues was significantly lower than that in normal ovarian tissues, but it was converse for PAI-1. In ovarian carcinoma, the expression of DLC1 was significantly associated with advanced FIGO stage, ascites and positive lymph node metastasis, whereas PAI-1 protein was closely related with advanced FIGO stage, poor histological differentiation and lymph node metastasis. The expression of DLC1 was negatively correlated with PAI-1 in ovarian carcinoma. Ovarian cancer patients with negative expression of DLC1 and positive expression of PAI-1 had the worst overall survival time compared to other patients. CONCLUSIONS: The expression of DLC1 and PAI-1 were closely related with the metastasis and invasion of ovarian carcinoma, only the combination of DLC1 and PAI-1 could serve as an independent prognostic factor of ovarian carcinoma.
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Authors | Fang Ren, Huirong Shi, Gong Zhang, Ruitao Zhang |
Journal | Journal of experimental & clinical cancer research : CR
(J Exp Clin Cancer Res)
Vol. 32
Pg. 60
(Aug 30 2013)
ISSN: 1756-9966 [Electronic] England |
PMID | 23988121
(Publication Type: Journal Article)
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Chemical References |
- DLC1 protein, human
- GTPase-Activating Proteins
- Plasminogen Activator Inhibitor 1
- Tumor Suppressor Proteins
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Topics |
- Adult
- Aged
- Blotting, Western
- Carcinoma, Ovarian Epithelial
- Cystadenocarcinoma, Mucinous
(genetics, metabolism, pathology)
- Cystadenocarcinoma, Serous
(genetics, metabolism, pathology)
- Female
- GTPase-Activating Proteins
(biosynthesis, genetics, metabolism)
- Humans
- Immunohistochemistry
- Middle Aged
- Neoplasms, Glandular and Epithelial
(genetics, metabolism, pathology)
- Ovarian Neoplasms
(genetics, metabolism, pathology)
- Plasminogen Activator Inhibitor 1
(biosynthesis, genetics, metabolism)
- Prognosis
- Transfection
- Tumor Suppressor Proteins
(biosynthesis, genetics, metabolism)
- Young Adult
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