Alginate lyase and ciprofloxacin co-immobilization on biopolymeric microspheres for cystic fibrosis treatment.

Abstract

A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.

• Authors: German A Islan, Valeria E Bosio, Guillermo R Castro
• Journal: Macromolecular bioscience (Macromol Biosci) Vol. 13 Issue 9 Pg. 1238-48 (Sep 2013) ISSN: 1616-5195 [Electronic] Germany
• PMID: 23966229 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Biopolymers
• Enzymes, Immobilized
• Solvents
• Ciprofloxacin
• Propylene Glycol
• Pectins
• Polysaccharide-Lyases
• poly(beta-D-mannuronate) lyase

Topics

• Biopolymers (chemistry)
• Ciprofloxacin (pharmacology, therapeutic use)
• Cystic Fibrosis (drug therapy)
• Enzymes, Immobilized (metabolism)
• Humans
• Hydrogen-Ion Concentration
• Kinetics
• Microscopy, Electron, Scanning
• Microspheres
• Pectins (chemistry)
• Polysaccharide-Lyases (metabolism)
• Propylene Glycol (chemistry)
• Solvents