Ingestion of low dose pyroglutamyl leucine improves dextran sulfate sodium-induced colitis and intestinal microbiota in mice.

Abstract

Inflammatory bowel diseases (IBD) are based on chronic inflammation in the gastrointestinal tract. We previously found anti-inflammatory peptide pyroGlu-Leu in the enzymatic hydrolysate of wheat gluten. The objective of present study is to elucidate improvement of colitis by oral administration of pyroGlu-Leu in an animal model. Acute colitis was induced by dextran sulfate sodium (DSS), and various concentrations of pyroGlu-Leu were administrated by oral gavage for 7 days. A dose of 0.1 mg/kg body weight/day showed the most significant improvement. The pyroGlu-Leu concentration was significantly increased 24 h after oral administration both in the small intestine and the colon compared with the baseline. It was 20-fold higher in the small intestine than the colon. Administration of pyroGlu-Leu normalized population of Bacteroidetes and Firmicutes in the colon. These results indicate that pyroGlu-Leu has a potential therapeutic effect against IBD at a practical dose.

• Authors: Sayori Wada, Kenji Sato, Ryoko Ohta, Eri Wada, Yukiho Bou, Miki Fujiwara, Tamami Kiyono, Eun Young Park, Wataru Aoi, Tomohisa Takagi, Yuji Naito, Toshikazu Yoshikawa
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 61 Issue 37 Pg. 8807-13 (Sep 18 2013) ISSN: 1520-5118 [Electronic] United States
• PMID: 23964746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Dipeptides
• pyroglutamylleucine
• Dextran Sulfate
• Pyrrolidonecarboxylic Acid

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage)
• Colitis (chemically induced, drug therapy, microbiology)
• Dextran Sulfate (adverse effects)
• Dipeptides (administration & dosage)
• Disease Models, Animal
• Humans
• Intestines (drug effects, microbiology)
• Male
• Mice
• Mice, Inbred C57BL
• Microbiota (drug effects)
• Pyrrolidonecarboxylic Acid (administration & dosage, analogs & derivatives)