Abstract |
To evaluate the potential role of DNA repair in bladder carcinogenesis, we performed an immunohistochemical analysis of expression of various DNA repair enzymes and γ-H2AX, a high-sensitivity marker of DNA double-strand breaks, in the urothelium of male F344 rats treated with N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN), a bladder-specific carcinogen. Our results clearly demonstrated that γ-H2AX aggregation was specifically generated in nuclei of bladder epithelial cells of BBN-treated rats, which was not found in untreated controls or mesenchymal cells. γ-H2AX-positive cells were detected not only in hyperplastic and neoplastic areas but also in the normal-like urothelium after BBN treatment. These data indicate that γ-H2AX has potential as a useful biomarker for early detection of genotoxicity in the rat urinary bladder. To the best of our knowledge, this is the first report demonstrating expression of γ-H2AX during bladder carcinogenesis.
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Authors | Takeshi Toyoda, Jun-Ichi Akagi, Young-Man Cho, Yasuko Mizuta, Saeko Onami, Isamu Suzuki, Kumiko Ogawa |
Journal | Journal of toxicologic pathology
(J Toxicol Pathol)
Vol. 26
Issue 2
Pg. 215-21
(Jun 2013)
ISSN: 0914-9198 [Print] Japan |
PMID | 23914065
(Publication Type: Journal Article)
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