Abstract |
Several Chinese herbs, namely, Pu-Gong-Ying, Gan-Cao, Chai-Hu, Mu-Xiang, Gua-Lou and Huang-Yao-Zi, are frequently used in complex traditional Chinese medicing formulas for breast hyperplasia and breast tumor therapy. The pharmacological effects of these Chinese herbs are all described as 'clearing heat-toxin and resolving masses' in traditional use. However, the chemical profiles of anti- breast cancer constituents in these herbs has not been investigated so far. In this study, a bioactivity-oriented screening platform, which was based on a human breast cancer MCF-7 cellular model, semi-preparative high performance liquid chromatography coupled with ultraviolet spectrophotometry and ultraperformance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometer, was developed to rapidly screen the six Chinese herbs. Two potential anti- breast cancer compounds, which were costunolide (Cos) and dehydrocostus lactone (Dehy), were identified in Mu-Xiang. Combination of the two compounds showed a synergism on inhibiting the proliferation of MCF-7 cells in vitro, which exhibits a potential application prospect for breast cancer therapy. This bioactivity-oriented screening strategy is rapid, economical, reliable and specific for screening potential anti- breast cancer compounds in traditional Chinese medicines.
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Authors | Zhang-xiao Peng, Yan Wang, Xue Gu, Yuan-yuan Wen, Chao Yan |
Journal | Biomedical chromatography : BMC
(Biomed Chromatogr)
Vol. 27
Issue 12
Pg. 1759-66
(Dec 2013)
ISSN: 1099-0801 [Electronic] England |
PMID | 23893711
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 John Wiley & Sons, Ltd. |
Chemical References |
- Antineoplastic Agents
- Drugs, Chinese Herbal
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Topics |
- Antineoplastic Agents
(chemistry, pharmacology)
- Breast Neoplasms
(drug therapy)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Chromatography, High Pressure Liquid
(methods)
- Drug Screening Assays, Antitumor
(methods)
- Drug Synergism
- Drugs, Chinese Herbal
(chemistry, pharmacology)
- Female
- Humans
- Plants, Medicinal
(chemistry)
- Spectrometry, Mass, Electrospray Ionization
(methods)
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