Abstract | BACKGROUND: METHODS: We developed a mouse model of wasp venom allergy by intraperitoneally (i.p.) injecting wasp venom into BALB/c mice twice on experimental day (day) 0 and 7. On day 20, a group of mice received an i.p. injection of mouse anti-IgE Ab as a pretreatment, and another group received rat anti-IgG1 Ab. On day 21, the animals were challenged by i.p. injection of wasp venom, and 30 min later, body temperature was measured and serum levels of leukotriene (LT) B4 and LTC4 were determined using enzyme immunoassay. RESULTS: The body temperature of mice treated with anti-IgE Ab and controls before and after wasp venom challenge was 37.8±0.2 vs 37.7± 0.3°C before challenge and 37.8±0.2 vs 37.1± 0.3°C after challenge, respectively, showing that anti-IgE Ab treatment significantly prevented body temperature from falling (p <0.05). Furthermore, anti-IgE Ab treatment reduced total serum IgE levels in the treated mice (42.2±15.9 pg/ml), compared with controls (105.9±23.1 pg/ml, p <0.05), and inhibited the secretion of LTC4 in the treated mice (32.0±18.8 pg/ml), but not in the controls (162.4±12.4 pg/ml, p <0.05), following challenge with wasp venom. CONCLUSION:
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Authors | Yoshihiko Ikeno, Hirokuni Hirata, Masafumi Arima, Yumeko Hayashi, Mineaki Watanabe, Naruo Yoshida, Kazuyuki Chibana, Kumiya Sugiyama, Yasutsugu Fukushima, Ikuo Akutsu, Takeshi Fukuda |
Journal | Asian Pacific journal of allergy and immunology
(Asian Pac J Allergy Immunol)
Vol. 31
Issue 2
Pg. 115-24
(Jun 2013)
ISSN: 0125-877X [Print] Thailand |
PMID | 23859410
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Wasp Venoms
- Leukotriene B4
- Leukotriene C4
- Immunoglobulin E
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Topics |
- Anaphylaxis
(blood, chemically induced, drug therapy, immunology)
- Animals
- Body Temperature
(drug effects, immunology)
- Disease Models, Animal
- Humans
- Immunoglobulin E
(immunology)
- Leukotriene B4
(blood, immunology)
- Leukotriene C4
(blood, immunology)
- Mice
- Rats
- Wasp Venoms
(toxicity)
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