Calyxin Y induces hydrogen peroxide-dependent autophagy and apoptosis via JNK activation in human non-small cell lung cancer NCI-H460 cells.

Abstract	Calyxin Y has been recently isolated from Alpinia katsumadai which has a folk use as an anti-tumor medicine. Calyxin Y induced caspase-dependent cell death in NCI-H460 cells, and concomitantly, provoked cytoprotective autophagy with the upregulation of critical Atg proteins. The cleavage of Atg proteins by caspases acted as a switch between autophagy and apoptosis induced by calyxin Y. Intracellular hydrogen peroxide (H2O2) production was triggered upon exposure to calyxin Y via the induction of autophagy and apoptosis. We provided evidence that activated JNK was upstream effectors controlling both autophagy and apoptosis in response to elevated H2O2. Therefore, our findings demonstrate that calyxin Y serves multiple roles as a promising chemotherapeutic agent that induces H2O2-dependent autophagy and apoptosis via JNK activation.
Authors	Chao Zhang, Lei Yang, Xiao-bing Wang, Jun-song Wang, Ya-di Geng, Chang-shui Yang, Ling-yi Kong
Journal	Cancer letters (Cancer Lett) Vol. 340 Issue 1 Pg. 51-62 (Oct 28 2013) ISSN: 1872-7980 [Electronic] Ireland
PMID	23811287 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Chemical References	ATG12 protein, human ATG5 protein, human Antineoplastic Agents, Phytogenic Autophagy-Related Protein 12 Autophagy-Related Protein 5 Chalcones Diarylheptanoids MAP1LC3A protein, human Microtubule-Associated Proteins Small Ubiquitin-Related Modifier Proteins calyxin Y Hydrogen Peroxide JNK Mitogen-Activated Protein Kinases Caspases
Topics	Antineoplastic Agents, Phytogenic (pharmacology) Apoptosis (drug effects) Autophagy (drug effects) Autophagy-Related Protein 12 Autophagy-Related Protein 5 Carcinoma, Non-Small-Cell Lung Caspases (metabolism) Cell Line, Tumor Cell Survival (drug effects) Chalcones (pharmacology) DNA Fragmentation Diarylheptanoids (pharmacology) Enzyme Activation Humans Hydrogen Peroxide (metabolism) Inhibitory Concentration 50 JNK Mitogen-Activated Protein Kinases (metabolism) Microtubule-Associated Proteins (metabolism) Small Ubiquitin-Related Modifier Proteins (metabolism) Up-Regulation

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