Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model.

Abstract

Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 μg/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-α, IL-1β and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye.

• Authors: Xinye Xiao, Pingping Luo, Hui Zhao, Jingyao Chen, Hui He, Yuxue Xu, Zhirong Lin, Yueping Zhou, Jianjiang Xu, Zuguo Liu
• Journal: Experimental eye research (Exp Eye Res) Vol. 115 Pg. 31-40 (Oct 2013) ISSN: 1096-0007 [Electronic] England
• PMID: 23792171 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Benzalkonium Compounds
• Interleukin-1beta
• Interleukin-6
• Ki-67 Antigen
• Tissue Extracts
• Tumor Necrosis Factor-alpha

Topics

• Administration, Topical
• Amnion (chemistry)
• Animals
• Benzalkonium Compounds (toxicity)
• Conjunctiva (drug effects, metabolism)
• Cornea (drug effects, metabolism)
• Disease Models, Animal
• Dry Eye Syndromes (chemically induced, metabolism, prevention & control)
• Female
• Goblet Cells (drug effects, metabolism)
• Humans
• In Situ Nick-End Labeling
• Interleukin-1beta (metabolism)
• Interleukin-6 (metabolism)
• Ki-67 Antigen (metabolism)
• Mice
• Mice, Inbred BALB C
• Tears (drug effects, metabolism)
• Tissue Extracts (therapeutic use)
• Tumor Necrosis Factor-alpha (metabolism)