MicroRNAs (
miRNAs) are small
RNA molecules that regulate posttranscriptional gene expression. Previous research has suggested that aberrant
miRNA expression often plays a critical role in many types of
cancer, including
lung cancer. However, the exact
miRNAs that are involved in pulmonary
carcinogenesis remain unclear. We investigated the
miRNA-based molecular changes that occur in
urethane-induced carcinogenicity and identified specific
miRNA deregulation in pulmonary
carcinogenesis induced by
urethane. In this study, we used a
lung cancer model in which Balb/c mice were exposed to
urethane via ip injection once a week for four consecutive weeks. The mice were then killed in weeks 6, 12, or 24. Two small
RNA libraries were constructed with the total
RNA from the lung
tumor and normal adjacent lung tissues of the
urethane-injected mice collected in week 24. Using Solexa sequencing, we identified a plethora of differentially expressed
miRNAs and predicted nine novel
miRNAs. Further analysis demonstrated the sustainable downregulation of miR-1a in the lung tissues in lung
carcinogenesis induced by
urethane. The levels of miR-1a were also reduced in the serum. Our findings indicate that
urethane exposure alters the expression of a cluster of
miRNAs. The simultaneous downregulation of miR-1a in lung tissues and serum in
urethane-induced pulmonary
carcinogenesis suggests that miR-1a is associated with
tumorigenesis.