Establishment of a human colorectal cancer cell line P6C with stem cell properties and resistance to chemotherapeutic drugs.

Abstract

AIM:

Cancer stem cells have the capacity to initiate and sustain tumor growth. In this study, we established a CD44(+) colorectal cancer stem cell line with particular emphasis on its self-renewal capacity, enhanced tumor initiation and drug resistance.

METHODS:

Fresh colon cancer and paired normal colon tissues were collected from 13 patients who had not received chemotherapy or radiotherapy prior to surgery. Among the 6 single-cell derived clones, only the P6C cell line was cultured for more than 20 passages in serial culture and formed holoclones with high efficiency, and then the stemness gene expression, colony formation, tumorigenicity and drug sensitivities of the P6C cell line were examined.

RESULTS:

Stemness proteins, including c-Myc, Oct3/4, Nanog, Lgr5, and SOX2, were highly expressed in the P6C cell line. Oct3/4-positive P6C cells mostly generated holoclones through symmetric division, while a small number of P6C cells generated meroclones through asymmetric division. P6C cells stably expressed CD44 and possessed a high capacity to form tumor spheres. A single cell-derived sphere was capable of generating xenograft tumors in nude mice. Compared to SW480 and HCT116 colorectal cancer cells, P6C cells were highly resistant to Camptothecin and 5-fluorouracil, the commonly used chemotherapeutic agents to treat colorectal cancers.

CONCLUSION:

We established a colorectal cancer stem cell line P6C with a high tumorigenic capacity and the characteristics of normal stem cells. It will benefit the mechanistic studies on cancer stem cells and the development of drugs that specifically target the cancer stem cells.

• Authors: Guan-hua Rao, Hong-min Liu, Bao-wei Li, Jia-jie Hao, Yan-lei Yang, Ming-rong Wang, Xiao-hui Wang, Jun Wang, Hai-jing Jin, Lei Du, Quan Chen
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 34 Issue 6 Pg. 793-804 (Jun 2013) ISSN: 1745-7254 [Electronic] United States
• PMID: 23736004 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Hyaluronan Receptors
• Fluorouracil
• Camptothecin

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Camptothecin (pharmacology)
• Cell Line, Tumor
• Colorectal Neoplasms (drug therapy, genetics, pathology)
• Drug Design
• Drug Resistance, Neoplasm
• Female
• Fluorouracil (pharmacology)
• Gene Expression Regulation, Neoplastic
• HCT116 Cells
• Humans
• Hyaluronan Receptors (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Molecular Targeted Therapy
• Neoplastic Stem Cells (metabolism)
• Xenograft Model Antitumor Assays (methods)