Caffeic acid phenethyl ester inhibits endothelial tissue factor expression.

Abstract

Caffeic acid phenethyl ester (CAPE) is a component of honeybee hives with various beneficial properties. Tissue factor (TF), the key trigger of thrombosis, is expressed in human endothelial cells. This study was designed to investigate whether CAPE modulates TF expression in human aortic endothelial cells (HAECs). Western blots and real-time polymerase chain reactions were performed. CAPE (10(-7)-10(-5) M) inhibited tumor necrosis factor (TNF)-α induced endothelial TF protein expression by 2.1-fold at 10(-5) M (p<0.0001). Similarly, TF surface activity was reduced (p<0.02). In contrast, TF mRNA expression, TF promoter activity, and mitogen-activated protein (MAP) kinase activation remained unaltered. In conclusion, CAPE inhibits TF protein expression and activity at the posttranscriptional level thereby exhibiting anti-thrombotic potential.

• Authors: Cathérine Gebhard, Barbara Elisabeth Stähli, Stephanie Largiadèr, Erik Walter Holy, Alexander Akhmedov, Giovanni Guido Camici, Thomas Felix Lüscher, Felix Christoph Tanner
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 36 Issue 6 Pg. 1032-5 ( 2013) ISSN: 1347-5215 [Electronic] Japan
• PMID: 23727925 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Caffeic Acids
• Fibrinolytic Agents
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• Thromboplastin
• Mitogen-Activated Protein Kinases
• caffeic acid phenethyl ester
• Phenylethyl Alcohol

Topics

• Aorta (cytology)
• Caffeic Acids (pharmacology)
• Cells, Cultured
• Endothelial Cells (drug effects, metabolism)
• Fibrinolytic Agents (pharmacology)
• Humans
• Mitogen-Activated Protein Kinases (metabolism)
• Phenylethyl Alcohol (analogs & derivatives, pharmacology)
• RNA, Messenger (metabolism)
• Thromboplastin (antagonists & inhibitors, genetics, metabolism)
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1 (genetics)