Abstract |
Phosphoglycerate kinase (PGK, EC 2.7.2.3), which is expressed specifically in sperm and spermatids, is an enzyme in the Embden-Meyerhof pathway that converts glucose to pyruvate. We developed an electrophoresis method to determine relative PGK-2 quantity and applied it to evaluate spermatogenesis activity. In the ethylene glycol monomethyl ether (EGME)-induced testicular toxicity, relative PGK-2 quantity had not decreased until 4 weeks of exposure. Mean relative PGK-2 quantities, defined as PGK-2 quantity over PGK-1 quantity in a pooled spleen sample (+/- SD) were: 1.43 +/- 0.32 for control animals (N = 10); 1.67 +/- 0.24 for the group exposed at 500 mg/kg for 5 days (N = 6); 1.85 +/- 0.58 for the group exposed at 500 mg/kg for 2 weeks (N = 6); 0.09 +/- 0.06 for the group exposed at 500 mg/kg for 4 weeks (N = 6); not detectable in animals exposed at 500 mg/kg for 5 weeks (N = 7); 0.208 +/- 0.103 for the group exposed at 250 mg/kg for 5 weeks (N = 6); and 1.35 +/- 0.38 for the group exposed at 125 mg/kg for 5 weeks (N = 6). These relative quantities showed a good correlation with sperm/spermatid counts (r = 0.823, p less than 0.01) and histological findings. These findings suggest that EGME has toxicity on primary spermatocytes and spermatogonia. In the case of sterility associated with a chromosomal abnormality ( chromosomal translocation between chromosome X and 16), relative PGK-2 quantity was not detected in any of the seven adult (12 weeks of age) mice, although many primary spermatocytes were detected by histological examination.(ABSTRACT TRUNCATED AT 250 WORDS)
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Authors | A Koizumi, N Hamade, M Arai, M Takatoku, W Yasuhiko, M Tsukada, S Kamiyama |
Journal | Archives of toxicology
(Arch Toxicol)
Vol. 64
Issue 3
Pg. 181-7
( 1990)
ISSN: 0340-5761 [Print] Germany |
PMID | 2372231
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Ethylene Glycols
- Isoenzymes
- Phosphoglycerate Kinase
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Topics |
- Animals
- Ethylene Glycols
(toxicity)
- Infertility, Male
(enzymology)
- Isoenzymes
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Phosphoglycerate Kinase
(analysis)
- Sperm Count
- Testis
(drug effects, enzymology)
- Translocation, Genetic
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