Decreased expression of miR-133a but not of miR-1 is associated with signs of heart failure in patients undergoing coronary bypass surgery.

Abstract

OBJECTIVES:

Coronary artery disease (CAD)-associated ischemic heart failure is characterized by dysregulated gene expression which is partly mediated by microRNAs (miRNAs). While the muscle-specific miR-1 and miR-133 are involved in cardiac development and hypertrophy, their role in heart failure resulting from CAD is unknown. We, therefore, tested the hypothesis that cardiac miR-1 and miR-133 expression is associated with signs of heart failure in patients undergoing coronary artery bypass grafting.

METHODS:

83 patients were included in this prospective study. Cardiac index and vascular pressures were measured under general anesthesia and the miRNA expression was quantified (RNase protection assay and real-time PCR) from samples of the right atrial myocardium.

RESULTS:

miR-133 expression decreased significantly with increased severity of heart failure, as indicated by a greater New York Heart Association (NYHA) functional class (p = 0.014) and increased pulmonary artery occlusion pressure (p = 0.045). Furthermore, patients with NT-proBNP concentrations >1,800 pg/ml showed a 25% decrease in miR-133 expression compared to patients with concentrations <300 pg/ml (p = 0.023). In contrast, no associations were detected for miR-1 expression.

CONCLUSIONS:

In surgical CAD patients, a decreased miR-133 expression is associated with variables characteristic of heart failure. This supports a role for miR-133 but not miR-1 in the adaption to and/or remodeling of the ischemic heart.

• Authors: Nina Danowski, Iris Manthey, Heinz Günther Jakob, Winfried Siffert, Jürgen Peters, Ulrich H Frey
• Journal: Cardiology (Cardiology) Vol. 125 Issue 2 Pg. 125-30 ( 2013) ISSN: 1421-9751 [Electronic] Switzerland
• PMID: 23711953 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 S. Karger AG, Basel.

Chemical References

• MIRN1 microRNA, human
• MIRN133 microRNA, human
• MicroRNAs

Topics

• Aged
• Coronary Artery Bypass
• Coronary Artery Disease (complications, metabolism, surgery)
• Heart Failure (etiology, metabolism)
• Humans
• MicroRNAs (metabolism)
• Middle Aged
• Myocardium (metabolism)