Abstract |
This study assessed whether endothelin-1 (ET-1) helps mediate postischemic acute kidney injury (AKI) progression to chronic kidney disease (CKD). The impact(s) of potent ETA or ETB receptor-specific antagonists ( Atrasentan and BQ-788, respectively) on disease progression were assessed 24 h or 2 weeks following 30 min of unilateral ischemia in CD-1 mice. Unilateral ischemia caused progressive renal ET-1 protein/ mRNA increases with concomitant ETA, but not ETB, mRNA elevations. Extensive histone remodeling consistent with gene activation and increased RNA polymerase II (Pol II) binding occurred at the ET-1 gene. Unilateral ischemia produced progressive renal injury as indicated by severe histologic injury and a 40% loss of renal mass. Pre- and post- ischemia or just postischemic treatment with Atrasentan conferred dramatic protective effects such as decreased tubule/microvascular injury, normalized tissue lactate, and total preservation of renal mass. Nuclear KI-67 staining was not increased by Atrasentan, implying that increased tubule proliferation was not involved. Conversely, ETB blockade had no protective effect. Thus, our findings provide the first evidence that ET-1 operating through ETA can have a critical role in ischemic AKI progression to CKD. Blockade of ETA provided dramatic protection, indicating the functional significance of these results.
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Authors | Richard A Zager, Ali C M Johnson, Dennis Andress, Kirsten Becker |
Journal | Kidney international
(Kidney Int)
Vol. 84
Issue 4
Pg. 703-12
(Oct 2013)
ISSN: 1523-1755 [Electronic] United States |
PMID | 23698233
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endothelin A Receptor Antagonists
- Endothelin B Receptor Antagonists
- Endothelin-1
- Oligopeptides
- Piperidines
- Pyrrolidines
- RNA, Messenger
- Receptor, Endothelin A
- Receptor, Endothelin B
- BQ 788
- Atrasentan
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Topics |
- Animals
- Atrasentan
- Disease Models, Animal
- Disease Progression
- Endothelin A Receptor Antagonists
- Endothelin B Receptor Antagonists
- Endothelin-1
(genetics, physiology)
- Kidney Failure, Chronic
(etiology, physiopathology)
- Male
- Mice
- Mice, Inbred Strains
- Oligopeptides
(pharmacology)
- Piperidines
(pharmacology)
- Pyrrolidines
(pharmacology)
- RNA, Messenger
(genetics)
- Receptor, Endothelin A
(drug effects, physiology)
- Receptor, Endothelin B
(drug effects, physiology)
- Renal Insufficiency, Chronic
(etiology, physiopathology)
- Reperfusion Injury
(complications, physiopathology)
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