The present study aimed to evaluate the role of
melanoma antigen family A (MAGEA) in gastric and
colorectal cancer cell lines and clinical tissue samples. we used 10 gastric and 9
colorectal cancer cell lines, 20 early-stage and 21 advanced-stage
gastric cancer tissues, 20 colon
adenomas and 19
colorectal cancer tissues. Real-time RT-PCR assay was used for the determination of MAGEA
mRNA levels. Western blot analysis and immunohistochemistry were used for the determination of MAGEA
protein levels in cell lines and tissues, respectively. Gastric and
colorectal cancer cell lines showed variable
mRNA expression levels of MAGEA. The MAGEA
protein was detected in 30% of
gastric cancer cell lines and in 22.2% of
colorectal cancer cell lines. There was a high correlation between
mRNA and
protein expression. Regarding the clinical samples, MAGEA expression was noted in 25, 28.6 and 31.6%, respectively in early-stage, advanced-stage
gastric cancer tissues and
colon adenocarcinoma, but was negative in the adjacent normal tissues of the stomach and colon as well as colon
adenoma. These results indicate that MAGEA is involved in the
carcinogenesis of gastric and
colorectal cancer and, therefore, can be used as a diagnostic marker to predict these
cancers.