Interaction of ω-3 polyunsaturated fatty acids with radiation therapy in two different colorectal cancer cell lines.

Abstract

BACKGROUND & AIMS:

This study aims at evaluating if docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) increases the efficacy of radiation therapy (RT) on two human colorectal cancer cell lines with different radio-sensitivity.

METHODS:

LS174T and HT-29 cells were treated with 20 or 50 μmol/L EPA or DHA followed by single X-ray RT of 0, 2 or 4 Gy, to evaluate cell survival, apoptosis, peroxide and malondialdehyde productions. Inflammation- and apoptosis-related proteins were analyzed by Western Blot. ANOVAs were used for statistical analysis.

RESULTS:

LS174T was more sensitive to RT than HT-29. DHA and to a lesser extent EPA increased cell death, apoptosis and peroxide production after RT in LS174T and to a lesser extent in HT-29 (p < 0.05). This was associated with increased expression of heat shock protein 70, decreased expression of NF-kB p65, COX-2 and Bcl-2 proteins.

CONCLUSIONS:

The effect of RT combination with DHA and to a lesser extent EPA was synergistic in the radio-sensitive LS174T cells, but additive in the radio-resistant HT-29 cells. This enhanced cytotoxicity was provoked at least partly by lipid peroxidation, which consequently modulated inflammatory response and induced apoptosis.

• Authors: Fang Cai, Olivier Sorg, Virginie Granci, Elena Lecumberri, Raymond Miralbell, Yves M Dupertuis, Claude Pichard
• Journal: Clinical nutrition (Edinburgh, Scotland) (Clin Nutr) Vol. 33 Issue 1 Pg. 164-70 (Feb 2014) ISSN: 1532-1983 [Electronic] England
• PMID: 23672803 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

Chemical References

• Fatty Acids, Omega-3
• NF-kappa B
• Malondialdehyde

Topics

• Apoptosis (drug effects, radiation effects)
• Cell Line, Tumor
• Cell Survival (drug effects, radiation effects)
• Colorectal Neoplasms (metabolism)
• Fatty Acids, Omega-3 (pharmacology)
• HT29 Cells
• Humans
• Lipid Peroxidation (drug effects, radiation effects)
• Malondialdehyde (metabolism)
• NF-kappa B (metabolism)
• Radiotherapy