Abstract | OBJECTIVE: DESIGN: Prospective, randomized animal study. SETTING: The study was performed at a university hospital laboratory. SUBJECTS: Thirty anesthetized adult male rats were included in the study. INTERVENTIONS: The study was performed in three groups: a sepsis/SIRS group (the S group), in which sepsis/SIRS was induced by cecal ligation and incision; a hemorrhage group (the H group), in which the rats were left without intervention for 4 h and bled 8 mL/kg thereafter; and a group that was left without intervention (the N group). Then, 4 h after baseline, all three groups were given an infusion of 0.9% NaCl (32 mL/kg) for 15 min. Baseline was defined as the time point when the surgical preparation was finished. MEASUREMENTS AND MAIN RESULTS: Plasma volumes were measured using I- albumin dilution technique at baseline, after 4 h, and 20 min after the end of infusion. The plasma volume-expanding effect 20 min after end of infusion was 0.6% ± 2.9% in the S group, 20% ± 6.4% in the H group, and 12% ± 11% in the N group, compared with just before start of infusion. CONCLUSIONS: The present study in rats showed that the plasma volume-expanding effect after an infusion of 0.9% NaCl was smaller in a septic/SIRS state than after hemorrhage and in a normal state. This indicates that the plasma volume-expanding effect of a crystalloid is dependent on pathophysiologic changes in sepsis/SIRS.
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Authors | Björn P Bark, Carl M Öberg, Per-Olof Grände |
Journal | Shock (Augusta, Ga.)
(Shock)
Vol. 40
Issue 1
Pg. 59-64
(Jul 2013)
ISSN: 1540-0514 [Electronic] United States |
PMID | 23649099
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plasma Substitutes
- Sodium Chloride
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Topics |
- Animals
- Hemorrhage
(physiopathology)
- Male
- Plasma Substitutes
(therapeutic use)
- Plasma Volume
(drug effects)
- Rats
- Sodium Chloride
(therapeutic use)
- Systemic Inflammatory Response Syndrome
(drug therapy)
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