Prime-O-glucosylcimifugin attenuates lipopolysaccharide-induced acute lung injury in mice.

Abstract

Prime-O-glucosylcimifugin is an active chromone isolated from Saposhnikovia root which has been reported to have various activities, such as anti-convulsant, anticancer, anti-inflammatory properties. The purpose of this study was to evaluate the effect of prime-O-glucosylcimifugin on acute lung injury (ALI) induced by lipopolysaccharide in mice. BALB/c mice received intraperitoneal injection of Prime-O-glucosylcimifugin 1h before intranasal instillation (i.n.) of lipopolysaccharide (LPS). Concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and interleukin (IL)-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Pulmonary histological changes were evaluated by hematoxylin-eosin, myeloperoxidase (MPO) activity in the lung tissue and lung wet/dry weight ratios were observed. Furthermore, the mitogen-activated protein kinases (MAPK) signaling pathway activation and the phosphorylation of IκBα protein were determined by Western blot analysis. Prime-O-glucosylcimifugin showed promising anti-inflammatory effect by inhibiting the activation of MAPK and NF-κB signaling pathway.

• Authors: Na Chen, Qianchao Wu, Gefu Chi, Lanan Wassy Soromou, Jinli Hou, Yanhong Deng, Haihua Feng
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 16 Issue 2 Pg. 139-47 (Jun 2013) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 23623941 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Lipopolysaccharides
• Monosaccharides
• NF-kappa B
• Xanthenes
• prim-O-glucosylcimifugin
• Mitogen-Activated Protein Kinases

Topics

• Acute Lung Injury (drug therapy, etiology, immunology, pathology)
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Bronchoalveolar Lavage Fluid (immunology)
• Cell Line
• Cytokines (immunology)
• Lipopolysaccharides
• Lung (immunology, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Mitogen-Activated Protein Kinases (immunology)
• Monosaccharides (pharmacology, therapeutic use)
• NF-kappa B (immunology)
• Xanthenes (pharmacology, therapeutic use)