Abstract | BACKGROUND AND AIMS: METHODS: Colonic mucosa biopsies were obtained from patients with ulcerative colitis and from matched controls. Inflammation was graded endoscopically and histologically. Esterified and non- esterified fatty acids were determined within the biopsies using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. RESULTS: Biopsy samples were collected from 69 UC patients (54 providing both inflamed and non-inflamed mucosa) and 69 controls. Inflamed mucosa had higher AA (p<0.001) and lower EPA (p<0.010) contents and a higher AA:EPA ratio (p<0.001). Inflamed mucosa also had higher docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) and lower linoleic acid (LA) and α- linolenic acid (α-LNA) contents (all p<0.001), compared to non-inflamed and controls. There were significant correlations between severity of inflammation and contents of AA, DPA and DHA (positive correlations) and of LA, α-LNA and EPA (negative correlations). CONCLUSIONS: Higher AA, AA:EPA ratio, DPA and DHA and lower LA, α-LNA and EPA are seen in inflamed mucosa in UC and correlate with severity of inflammation. This suggests an alteration in fatty acid metabolism in the inflamed gut mucosa, which may offer novel targets for intervention and should be considered if nutritional strategies are used.
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Authors | Daniel S Pearl, Mojgan Masoodi, Michael Eiden, Janine Brümmer, Darren Gullick, Tricia M McKeever, Mark A Whittaker, Harriet Nitch-Smith, James F Brown, Janis K Shute, Graham Mills, Philip C Calder, Timothy M Trebble |
Journal | Journal of Crohn's & colitis
(J Crohns Colitis)
Vol. 8
Issue 1
Pg. 70-9
(Jan 2014)
ISSN: 1876-4479 [Electronic] England |
PMID | 23619007
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 European Crohn's and Colitis Organisation. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Fatty Acids, Omega-3
- Fatty Acids, Omega-6
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Topics |
- Adult
- Biological Availability
- Case-Control Studies
- Colitis, Ulcerative
(metabolism, pathology)
- Colon
(metabolism, pathology)
- Diet
- Esterification
- Fatty Acids, Omega-3
(chemistry, pharmacokinetics)
- Fatty Acids, Omega-6
(chemistry, pharmacokinetics)
- Female
- Humans
- Intestinal Mucosa
(metabolism, pathology)
- Male
- Middle Aged
- Prospective Studies
- Severity of Illness Index
- Surveys and Questionnaires
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