Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa.

Abstract

Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications.

• Authors: Xiaoqin Wang, Xuewu Zhang
• Journal: Biotechnology progress (Biotechnol Prog) 2013 May-Jun Vol. 29 Issue 3 Pg. 681-7 ISSN: 1520-6033 [Electronic] United States
• PMID: 23606619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 American Institute of Chemical Engineers.

Chemical References

• Antineoplastic Agents
• Nanocapsules
• Peptide Fragments
• Peptides
• Plant Extracts
• Plant Proteins
• Endopeptidases

Topics

• Antineoplastic Agents (chemistry, isolation & purification, pharmacokinetics, pharmacology)
• Chlorella (chemistry)
• Chromatography, Gel
• Endopeptidases
• Hep G2 Cells
• Humans
• Nanocapsules (chemistry)
• Particle Size
• Peptide Fragments (chemistry, isolation & purification, pharmacokinetics, pharmacology)
• Peptides (chemistry, isolation & purification, pharmacokinetics, pharmacology)
• Plant Extracts (chemistry)
• Plant Proteins (chemistry, isolation & purification, pharmacokinetics, pharmacology)