SFMBT1 functions with LSD1 to regulate expression of canonical histone genes and chromatin-related factors.

Abstract	SFMBT1 (Scm [Sex comb on midleg] with four MBT [malignant brain tumor] domains 1) is a poorly characterized mammalian MBT domain-containing protein homologous to Drosophila SFMBT, a Polycomb group protein involved in epigenetic regulation of gene expression. Here, we show that SFMBT1 regulates transcription in somatic cells and during spermatogenesis through the formation of a stable complex with LSD1 and CoREST. When bound to its gene targets, SFMBT1 recruits its associated proteins and causes chromatin compaction and transcriptional repression. SFMBT1, LSD1, and CoREST share a large fraction of target genes, including those encoding replication-dependent histones. Simultaneous occupancy of histone genes by SFMBT1, LSD1, and CoREST is regulated during the cell cycle and correlates with the loss of RNA polymerase II at these promoters during G2, M, and G1. The interplay between the repressive SFMBT1-LSD1-CoREST complex and RNA polymerase II contributes to the timely transcriptional regulation of histone genes in human cells. SFMBT1, LSD1, and CoREST also form a stable complex in germ cells, and their chromatin binding activity is regulated during spermatogenesis.
Authors	Jin Zhang, Roberto Bonasio, Francesco Strino, Yuval Kluger, J Kim Holloway, Andrew J Modzelewski, Paula E Cohen, Danny Reinberg
Journal	Genes & development (Genes Dev) Vol. 27 Issue 7 Pg. 749-66 (Apr 01 2013) ISSN: 1549-5477 [Electronic] United States
PMID	23592795 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References	Chromatin Co-Repressor Proteins Histones Nerve Tissue Proteins RCOR1 protein, human Repressor Proteins SFMBT1 protein, human Sfmbt1 protein, mouse Histone Demethylases KDM1a protein, mouse KDM1A protein, human Oxidoreductases, N-Demethylating
Topics	Animals Chromatin (genetics, metabolism) Co-Repressor Proteins Gene Expression Regulation Genome HEK293 Cells HeLa Cells Histone Demethylases (genetics, metabolism) Histones (genetics, metabolism) Humans Male Mice Mice, Inbred C57BL Nerve Tissue Proteins (metabolism) Oxidoreductases, N-Demethylating (metabolism) Protein Binding Protein Stability Protein Transport Repressor Proteins (genetics, metabolism) Sf9 Cells Spermatogenesis (genetics) Testis (metabolism)

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