MicroRNA-21 and -146b are involved in the pathogenesis of murine viral myocarditis by regulating TH-17 differentiation.

Abstract

Viral myocarditis (VMC) is a common cardiovascular disease, and microRNAs (miRNAs) have been postulated to be involved in its pathology. Using microarrays, we observed that miRNA-21 and -146b were upregulated in a murine model of VMC. We also found that miRNA-451 was downregulated. In vivo silencing of miRNA-21 and -146b resulted in less-severe VMC. Overexpression of miRNA-451 did not ameliorate the severity of VMC. Further work revealed that inhibition of miRNA-21 and -146b decreased the expression levels of Th17 and RORγt . Overexpression of miRNA-451 had no effect on IL-17 and RORγt expression. Inhibition of miRNA-21 and -146b might ameliorate myocardium inflammation by mediating downregulation of RORγt expression, indicating that these miRNAs are involved in the pathogenesis of murine VMC.

• Authors: Yan Li Liu, Weifeng Wu, Yimin Xue, Mengsha Gao, Yuluan Yan, Qing Kong, Yu Pang, Fan Yang
• Journal: Archives of virology (Arch Virol) Vol. 158 Issue 9 Pg. 1953-63 (Sep 2013) ISSN: 1432-8798 [Electronic] Austria
• PMID: 23588407 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN146 microRNA, human
• MIRN21 microRNA, human
• MicroRNAs
• Nuclear Receptor Subfamily 1, Group F, Member 3
• Rorc protein, mouse

Topics

• Animals
• Cell Differentiation (drug effects)
• Cell Line
• Coxsackievirus Infections (genetics, physiopathology, virology)
• Disease Models, Animal
• Enterovirus B, Human (pathogenicity)
• Gene Expression Profiling
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• MicroRNAs (genetics, metabolism, pharmacology)
• Myocarditis (genetics, physiopathology, virology)
• Myocardium (metabolism, pathology)
• Nuclear Receptor Subfamily 1, Group F, Member 3
• Oligonucleotide Array Sequence Analysis
• Th17 Cells (cytology)
• Up-Regulation