Proteomic analysis of plasma of patients with left ventricular remodeling after myocardial infarction: usefulness of SELDI-TOF.

Abstract

SELDI-TOF and depletion of major blood proteins is one of the most promising approaches for accessing low-abundance biomarkers. The use of combinatorial peptide ligand library (CPLL) for selecting the low-abundance proteins and of liquid-phase isoelectric focusing for purifying the corresponding proteins was tested in plasma or serum from patients with myocardial infarction (MI). Here, we describe the SELDI profiling of CPLL-treated plasma to select low-abundance proteins in plasma and the strategy for purification and mass spectrometry identification. This approach shows the potential to select and identify candidate biomarkers in patients with left ventricular remodeling after MI.

• Authors: Florence Pinet
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1000 Pg. 201-7 ( 2013) ISSN: 1940-6029 [Electronic] United States
• PMID: 23585094 (Publication Type: Journal Article)

Chemical References

• Blood Proteins
• Peptide Library

Topics

• Analytic Sample Preparation Methods
• Blood Proteins (chemistry, isolation & purification, metabolism)
• Humans
• Myocardial Infarction (blood, physiopathology)
• Peptide Library
• Proteomics (methods)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)
• Ventricular Remodeling